Competency Assessment: Diagnostic Methods for Detection of Cryptosporidium, Microsporidia, and Toxoplasma in Bronchoalveolar Lavage Samples

Background: Protozoa have the ability to replace human lung. Over recent years, incidence of pulmonary infections caused by these microorganisms has increased, particularly in individuals with an immunodeficiency. The use appropriate diagnostic methods is important identification parasites secretions. Objectives: present study aimed evaluate and compare PCR-based gold standard method detect three pathogenic protozoa, including Toxoplasma, Cryptosporidium, Microsporidia bronchoalveolar lavage (BAL) samples obtained from immunocompromised patients chronic obstructive disease. Methods: A BAL sample immunodeficient suffering disease (COPD) was examined direct microscopy PCR methods. Results: In this study, we 64 immunodeficiency accompanied COPD. were not identified samples. Direct nine cases Toxoplasma respectively. However, molecular two infection parasites. Conclusions: Determining for dependent on factors, such as type specimen parasite. Based results microscopic optimal Cryptosporidium